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  • Title: [Determination of akebia saponin D in root of Dipacus asperoides by HPLC].
    Author: Tan HG, Lin S, Zhang QW, Ji L.
    Journal: Zhongguo Zhong Yao Za Zhi; 2006 May; 31(9):726-7, 739. PubMed ID: 17048676.
    Abstract:
    OBJECTIVE: To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides. METHOD: An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm. RESULT: The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively. CONCLUSION: This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.
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