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  • Title: HuGE, a novel GFP-actin-expressing mouse line for studying cytoskeletal dynamics.
    Author: Gurniak CB, Witke W.
    Journal: Eur J Cell Biol; 2007 Jan; 86(1):3-12. PubMed ID: 17049405.
    Abstract:
    Analysis of actin remodeling in live cells and tissues has become an increasingly important tool to study actin-dependent cellular processes. To facilitate these experiments in the mouse we have generated a GFP-actin-expressing line (huGE) by knock-in of the GFP-actin gene into the profilin 1 locus. Here we show that GFP-actin is expressed throughout embryonic development and in all tissues except skeletal muscle, in a pattern similar to profilin 1. Particularly high expression of GFP-actin was observed in bone marrow and all blood cells. The GFP-actin fusion protein is functional as shown by its co-localization with endogenous actin in F-actin-rich structures. Therefore, the huGE mouse line provides a novel tool to monitor actin dynamics in mouse embryos and a wide range of organs.
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