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  • Title: Activation of alpha-myosin heavy chain gene expression by cAMP in cultured fetal rat heart myocytes.
    Author: Gupta MP, Gupta M, Stewart A, Zak R.
    Journal: Biochem Biophys Res Commun; 1991 Feb 14; 174(3):1196-203. PubMed ID: 1705119.
    Abstract:
    The effect of cAMP on cardiac myosin heavy chain (MHC) gene expression in primary cultures of 18-day-old fetal rat heart myocytes was investigated. When myocytes were treated with either 10 microM forskolin or 1 mM 8-bromo-cAMP for 48 h, the relative amount of the V1 to -V3 myosin isoform ratio increased 3-fold. The abundance of alpha-MHC mRNA was also increased 3-to-4-fold in forskolin treated vs control cells. However, no appreciable change was observed in the level of beta-MHC mRNA. In addition, a 70% increase in the transcription rate of the cardiac MHC gene was observed by nuclear run-on assay following treatment of cells with 10 microM forskolin for 12 h. These results demonstrate the preferential induction of alpha-MHC mRNA by cAMP which is, in part, mediated by transcriptional activation of the gene.
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