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  • Title: Microemulsion electrokinetic chromatography separation by using hexane-in-water microemulsions without cosurfactant: comparison with MEKC.
    Author: Nozal L, Arce L, Simonet BM, Ríos A, Valcárcel M.
    Journal: Electrophoresis; 2006 Nov; 27(22):4439-45. PubMed ID: 17054085.
    Abstract:
    A new hexane-in-water microemulsion was investigated as buffer in microemulsion EKC (MEEKC). At difference with other microemulsions, the addition of cosurfactant was not necessary to stabilize the microemulsion. The proposed microemulsion was successfully used to achieve electrophoretic separation of seven antibiotics including nitroimidazoles, cephapirin and tetracyclines. Selectivity and separation efficiency achieved in MEEKC were compared with MEKC. MEEKC technique proved to be more efficient than MEKC for performing the separation of the analytes and the presence of microemulsions was found to be critical to achieve the separation of tetracyclines. The proposed microemulsion also points out that solvents with high volatility, such as hexane, can be stabilized and used as a microemulsion of SDS.
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