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  • Title: [Determination of indirubin and indigo in Baphicacanthus cusia (Nees) Bremek by HPLC].
    Author: Hou HC, Liang SZ.
    Journal: Zhong Yao Cai; 2006 Jul; 29(7):681-2. PubMed ID: 17059007.
    Abstract:
    OBJECTIVE: To establish a method for content determination of indirubin and indigo in Baphicacanthus cusia (Nees) Bremek. METHOD: HPLC was used with C18 column, methanol-water (75:25) as mobile phase and detecting wavelength 289 nm. RESULTS: The range linear curve was 3.875 approximately 23.25 microg/ml (indirubin), 3.05 approximately 18.3 microg/ml (indigo). The average recovery was 96.9%, RSD =3.5%, n =5 (indirubin); 96.9% RSD = 2.8%, n = 5 (indigo). CONCLUSION: The method is proper for the quality control of Baphicacanthus cusia (Nees) Bremek.
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