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  • Title: Sequence characterized amplified region (SCAR) analysis on DNA from the three medicinal Echinacea species.
    Author: Adinolfi B, Chicca A, Martinotti E, Breschi MC, Nieri P.
    Journal: Fitoterapia; 2007 Jan; 78(1):43-5. PubMed ID: 17067760.
    Abstract:
    In our previous study, RAPD (Random Amplified Polymorphic DNA) analysis revealed species-specific markers for three medicinal Echinacea species (Asteraceae): E. angustifolia DC., E. pallida (Nutt.) Nutt. and E. purpurea (L.) Moench. In the present work, we have converted a RAPD marker (750 bp) for E. purpurea into a SCAR (Sequence Characterized Amplified Region) marker. SCAR-PCR, in fact, revealed the expected amplicon (330 bp) only in E. purpurea and not in the other two species, giving further evidence for differences in medicinal Echinacea spp. genome and confirming a greater similarity between E. pallida and angustifolia.
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