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  • Title: Engineering of GAL1 promoter-driven expression system with artificial transcription factors.
    Author: Park KS, Kim JS.
    Journal: Biochem Biophys Res Commun; 2006 Dec 15; 351(2):412-7. PubMed ID: 17069762.
    Abstract:
    We isolated and characterized artificial transcription factors (ATFs) that functionally activate GAL1 promoter in yeast. These ATFs transformed the yeast galactose-dependent GAL1 promoter system into a galactose-independent one. The ATFs were identified by screening a combinatorial library of zinc finger-containing transcription factors for components that activated the transcription of a reporter gene under the control of a truncated GAL1 promoter from which the GAL4p-binding sites were deleted. We also showed that these ATFs activate transcription from GAL1 promoter by binding directly to specific sequence elements in the promoter.
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