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  • Title: Electron paramagnetic resonance (EPR) spin trapping of biological nitric oxide.
    Author: Kleschyov AL, Wenzel P, Munzel T.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2007 May 15; 851(1-2):12-20. PubMed ID: 17070113.
    Abstract:
    Nitric oxide (NO) is a free radical species with multiple physiological functions. Because of low concentrations and short half-life of NO, its direct measurement in living tissues remains a difficult task. Electron paramagnetic resonance (EPR) spin trapping is probably one of the best suitable platforms for development of new methods for quantification of biological NO. The most reliable EPR-based approaches developed so far are based on the reaction of NO with various iron complexes, both intrinsic and exogenously applied. This review is focused on the current state and perspectives of EPR spin trapping for experimental and clinical NO biology.
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