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  • Title: Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1.
    Author: Singh RS, Sooch BS, Puri M.
    Journal: Bioresour Technol; 2007 Sep; 98(13):2518-25. PubMed ID: 17071081.
    Abstract:
    A newly isolated strain of Kluyveromyces marxianus YS-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, CaCl2 and sodium dodecyl sulphate (SDS). Fermentation medium pH 6.5, cultivation temperature 30 degrees C and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 IU/ml) with a fermentation time of 72 h at shake flask level. Raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was optimum for bioreactor studies. Maximum enzyme production (55.4 IU/ml) was obtained at an agitation rate of 200 rpm and aeration of 0.75 vvm in a stirred tank reactor with a fermentation time of 60 h.
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