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  • Title: Targeting vector construction by yeast artificial chromosome modification.
    Author: Murray PJ.
    Journal: Methods Mol Biol; 2006; 349():127-37. PubMed ID: 17071979.
    Abstract:
    Mouse yeast artificial chromosomes (YACs) are useful platforms for manipulation of targeting vector design and construction, particularly in circumstances where polymerase chain reaction-mediated amplification of targeting arms proves fruitless or the cloned DNA is inherently unstable. This chapter describes a simple procedure where YACs can be modified to produce targeting vectors of various sizes.
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