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  • Title: [Construction and preliminary application of a human natural phage antibody library with diversity].
    Author: Wang J, Luo WX, Li LF, Chen YW, Wang MQ, Zhang J, Xia NS.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2006 Nov; 22(6):786-9, 793. PubMed ID: 17077021.
    Abstract:
    AIM: To construct a human natural phage single-chain antibody (scFv) library with diversity. METHODS: V(H) and V(L) genes were amplified by RT-PCR and hemi-PCR from peripheral blood lymphocytes of healthy persons. The V genes were assembled to form scFv by overlap PCR and cloned into phagemid pCANTAB-5E, and then transformed into E. coli TG1 by electroporation to construct a human natural phage scFv library. The diversity and gene family of antibody gene were analysed by sequencing and the specific antibodies against various antigens were screened through bio-panning. RESULTS: A human natural phage scFv library with diversity and 2x10(8) sink size was constructed successfully. The specific human scFvs against 5 antigens were obtained by bio-panning. CONCLUSION: A human natural phage scFv library with diversity is constructed successfully and can be applied to human antibody preparation.
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