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  • Title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapA (Rv2753c) from Mycobacterium tuberculosis.
    Author: Kefala G, Weiss MS.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2006 Nov 01; 62(Pt 11):1116-9. PubMed ID: 17077492.
    Abstract:
    Dihydrodipicolinate synthase from Mycobacterium tuberculosis (DHDPS, DapA, Rv2753c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in a monoclinic crystal form. Preliminary diffraction data analysis suggests the presence of two independent tetramers in the asymmetric unit in almost the same relative orientation.
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