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  • Title: CEC with monolithic poly(styrene-divinylbenzene-vinylsulfonic acid) as the stationary phase.
    Author: Huang HY, Lin HY, Lin SP.
    Journal: Electrophoresis; 2006 Dec; 27(23):4674-81. PubMed ID: 17080486.
    Abstract:
    In this study, poly(styrene-divinylbenzene-vinylsulfonic acid) monolithic columns, prepared by singlestep in situ polymerization, were first used as separation columns for CEC in which vinylsulfonic acid was employed as the charge-bearing monomer. The effects of the polymerization conditions (compositions of monomer and porogenic solvent, and polymerization time) on the chromatographic property of the polystyrene-based monolithic column were examined. Furthermore, an increase in water content of the porogenic solvent markedly altered the retention behaviors of these analytes, where the monolithic column was able to be produced in a higher ratio of vinylsulfonic acid. Finally, the study demonstrated that acidic analytes could be successfully separated by the polystyrene-based monolithic column under an acidic mobile phase.
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