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  • Title: Comparison of ELISA and immunoassays for measurement of IgG and IgM antibody to West Nile virus in human sera against virus neutralisation.
    Author: Niedrig M, Sonnenberg K, Steinhagen K, Paweska JT.
    Journal: J Virol Methods; 2007 Jan; 139(1):103-5. PubMed ID: 17084464.
    Abstract:
    Two commercial assays for the detection of IgG antibody to West Nile virus (WNV), an indirect enzyme-linked immunosorbent assay (I-ELISA) and indirect fluorescent antibody test (IFAT), were evaluated against the virus neutralisation test. Excellent agreement with the virus neutralisation was obtained with both tests, i.e., 99.5% by I-ELISA and 100% by IFAT. The well-known serological cross-reactivity within the family of the Flaviviridae was analysed using sera with known antibodies against dengue virus, tick borne encephalitis virus and yellow fever virus. IgM and/or IgG positive sera were examined for reactivity by WNV-ELISA and WNV-IFAT. While cross-reactivity between 0 and 18.2% was recorded with IgM positive sera, there was extensive cross-reactivity of 15.7-100% with IgG positive sera.
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