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  • Title: Formation and rapid export of the monochlorobimane-glutathione conjugate in cultured rat astrocytes.
    Author: Waak J, Dringen R.
    Journal: Neurochem Res; 2006 Dec; 31(12):1409-16. PubMed ID: 17089195.
    Abstract:
    Monochlorobimane (MCB) is often used to visualize glutathione (GSH) levels in cultured cells, since it is quickly converted to a fluorescent GSH conjugate (GS-MCB). To test for consequences of MCB application on the GSH metabolism of astrocytes, we have studied rat astrocyte-rich primary cultures as model system. MCB caused a concentration dependent rapid decrease in the cellular GSH content. Simultaneously, a transient accumulation of GS-MCB in the cells was observed with a maximal content 5 min after MCB application. The cellular accumulation was followed by a rapid release of GS-MCB into the medium with a maximal initial export rate of 27.9 +/- 6.5 nmol h(-1) mg protein(-1). Transporters of the family of multidrug resistance proteins (Mrps) are likely to be involved in this export, since the Mrp inhibitor MK571 lowered the export rate by 60%. These data demonstrate that, due to its rapid export from astrocytes, GS-MCB is only under well-defined conditions a reliable indicator of the cellular GSH concentration and that MK571 can be used to maintain maximal GS-MCB levels in astrocytes.
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