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  • Title: Multiple thermal discontinuities in glucose-6-phosphatase activity.
    Author: Grinna LS.
    Journal: Biochim Biophys Acta; 1975 Oct 22; 403(2):388-92. PubMed ID: 170970.
    Abstract:
    The temperature dependence of glucose-6-phosphatase (D-glucose-6-phosphate phosphohydrolase EC 3.1.3.9) was studied in rat liver and kidney microsomal fractions. Arrhenius plots were non-linear and showed four distinct discontinuities in enzyme activity over the temperature range 2-41 degrees C. The discontinuities occurred at approx. 39, 30, 20 and 12 degrees C in the liver and were similar to this in the kidney. Changes in the energy of activation for the enzyme were noted at approx. 20 degrees C in both tissues. The multiple discontinuities in glucose-6-phosphatase activity are viewed as a reflection of complex reorganization and/or change in physical state of the membrane components, primarily lipid.
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