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  • Title: Production of penicillin acylase by a recombinant Escherichia coli using cheese whey as substrate and inducer.
    Author: De León-Rodríguez A, Rivera-Pastrana D, Medina-Rivero E, Flores-Flores JL, Estrada-Baltazar A, Ordóñez-Acevedo LG, de la Rosa AP.
    Journal: Biomol Eng; 2006 Dec; 23(6):299-305. PubMed ID: 17097344.
    Abstract:
    Cheese whey (CW) is the major subproduct from cheese manufacturing and it is considered as a waste pollutant since its high content of lactose. In this work a fermentation process for the production of penicillin acylase (PA) by a recombinant Escherichia coli and using CW as unique carbon source and inducer was developed. A design factorial 3(2) was used to evaluate the influence of independent variables (dissolved oxygen and CW concentration) on the ability of E. coli W3110/pPA102 to produce PA. Maximum specific PA activity of 781 U g(-1) was attained at 5 g L(-1) of CW and 3% dissolved oxygen. The results showed that CW can be used successfully as unique carbon source and inducer for the production of recombinant proteins using constructions driven by the lac promoter and this way reducing the discharges of that pollutant to the environment.
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