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  • Title: Evaluation of gamma-interferon kinetics in HSV-1 infected mice in different days post infection (in vivo) and post re-stimulation (in vitro).
    Author: Arefian E, Bamdad T, Soleimanjahi H, Sabahi F, Akhoond MR, Parsania M, Ghaemi A.
    Journal: Comp Immunol Microbiol Infect Dis; 2007 Jan; 30(1):1-9. PubMed ID: 17098286.
    Abstract:
    Gamma interferon (IFN-gamma) is among the most important immune factors for limiting of herpes simplex virus (HSV) infections. However, our knowledge about the kinetics of IFN-gamma production after HSV infection is limited. The present study examines the kinetics of IFN-gamma expression following secondary infection with HSV-1. Using semiquantitative RT-PCR assay, the expression of IFN-gamma in spleen lymphocytes was significantly detected on 14 days but not 7 days after intraperitoneal inoculation of HSV-1, while ELISA detected IFN-gamma on both days. At various hours after in vitro re-stimulation of spleen cells, RT-PCR showed a decreasing pattern of mRNA transcripts, whereas, ELISA assayed an increasing amount of secreted protein through the experiment. Despite the contrast results of ELISA and RT-PCR, regarding the short half-life of mRNA, the data are in correlation with each other and need to interpret.
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