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  • Title: Preparation of lutropin with acetyl or acetimidinyl substituents on the amino groups of the beta-subunit.
    Author: Liu WK, Ward DN.
    Journal: Biochim Biophys Acta; 1975 Oct 20; 405(2):522-6. PubMed ID: 170984.
    Abstract:
    The free amino groups in ovine lutropin beta subunit were acylated with acetic anhydride and methyl acetimidinate-HCl to produce the corresponding acetyl and acetimidinyl ovine lutropin beta derivative. These two derivatives recombined with ovine lutropin alpha as well as native ovine lutropin beta, but produced lutropin derivatives which were 33-50% less active than the ovine lutropin alpha + ovine lutropin beta in biological assays.
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