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  • Title: [Introduction of transpozon Tn9 to endogenic plasmids of Erwinia carotovora during lysogenization of cells by coliphage P1].
    Author: Sergeeva ZhIu, Burova LM, Tovkach FI.
    Journal: Mikrobiol Z; 2006; 68(4):34-9. PubMed ID: 17100326.
    Abstract:
    It has been shown that phage P1 of Escherichia coli is able not only to lysogenize the cells of Erwinia carotovora but also to be a source of transpozon Tn9 for mutagenesis of cryptic plasmids of this important phytopathogen. Since the amount of the introduction in one of plasmids pCA25::Tn9 is 3.8 kb, at an average, it is supposed that Tn9 is inherited as a double tandem structure. A convenient method is offered for selecting bacterial clones carrying the plasmid pCA25::Tn9 which is based on counterselection of P1-lysogens at high concentrations of chloramphenicol - 100-120 microg/ml.
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