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  • Title: [A method of determining heparin in blood plasma based on its antithrombin activity].
    Author: Taktashev RE, Suvorova AV, Miller VE, Kolesnikova OI.
    Journal: Lab Delo; 1990; (12):59-61. PubMed ID: 1710698.
    Abstract:
    The suggested method for measuring blood plasma heparin is based on heparin ability to enhance antithrombin activity of antithrombin III (AT-III), the major Xa and thrombin inhibitor. The method consists in measurement of blood plasma AT-III activity in the presence and absence of protamine sulfate that destroys the heparin--AT-III complex. Heparin content in U/ml is determined from the difference in the activities of heparin--AT-III complex and AT-III proper activity represented on the calibration curve. The method is sufficiently sensitive, it permits registration of heparin concentrations in a wide band (from 0.01 U/ml to 0.75 U/ml of plasma).
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