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  • Title: Label-free immunosensor for prostate-specific antigen based on single-walled carbon nanotube array-modified microelectrodes.
    Author: Okuno J, Maehashi K, Kerman K, Takamura Y, Matsumoto K, Tamiya E.
    Journal: Biosens Bioelectron; 2007 Apr 15; 22(9-10):2377-81. PubMed ID: 17110096.
    Abstract:
    We have fabricated a label-free electrochemical immunosensor using microelectrode arrays modified with single-walled carbon nanotubes (SWNTs). Label-free detection of a cancer marker, total prostate-specific antigen (T-PSA), was carried out using differential pulse voltammetry (DPV). The current signals, derived from the oxidation of tyrosine (Tyr), and tryptophan (Trp) residues, increased with the interaction between T-PSA on T-PSA-mAb covalently immobilized on SWNTs. The selectivity of our biosensor was challenged using bovine serum albumin (BSA) as the target protein. The detection limit for T-PSA was determined as 0.25 ng/mL. Since the cut-off limit of T-PSA between prostate hyperplasia and cancer is 4 ng/mL, the performance of our label-free electrochemical immunosensor seems promising for further clinical applications.
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