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  • Title: Fluorescence lifetime imaging microscopy: two-dimensional distribution measurement of fluorescence lifetime.
    Author: Fujiwara M, Cieslik W.
    Journal: Methods Enzymol; 2006; 414():633-42. PubMed ID: 17110215.
    Abstract:
    A newly developed fluorescence lifetime imaging microscopy (FLIM) system has combined the high-temporal resolution of a streak camera with the high-spatial resolution of a microscope to obtain a two-dimensional distribution of fluorescence lifetimes within living cells. The temporal resolution is as short as 20 ps. The effective field of view is 48 x 45 mum with 0.2 mum resolution using a 60x water immersion objective. Image acquisition time is as short as 3 s per image. Measured and published values of lifetime for standard fluophores are shown with good agreement. Examples of FLIM and fluorescence resonance energy transfer images are presented.
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