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  • Title: Regulation and characterization of the polarity of cells embedded in a reconstructed basement matrix using a three-dimensional micro-culture system.
    Author: Torisawa YS, Nashimoto Y, Yasukawa T, Shiku H, Matsue T.
    Journal: Biotechnol Bioeng; 2007 Jun 15; 97(3):615-21. PubMed ID: 17115450.
    Abstract:
    Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was dependent on the surrounding conditions (2-D, collagen gel, or Matrigel), whereas the respiratory activity of a single cell (F(c)) was almost identical under different culture conditions. The F(c) value changed with cellular polarity. The F(c) value for the S-1 cells was observed to decrease slightly, whereas that of the T4-2 cells increased 2 days after cultivation in the microstructures within the Matrigel. However, when the T4-2 cells were cultured in the presence of tyrphostin AG 1478 (T4-2 tyr) to inhibit epidermal growth factor (EGF) signaling, the F(c) value decreased slightly and remained almost constant for an additional 1 week; this was similar to the behavior of the S-1 cells. Further, fluorescence images showed that the T4-2 tyr cells formed polar structures that were similar to those formed by the S-1 cells whereas the T4-2 cells did not form such structures. These results indicate that cellular polarity can be assessed by measuring cellular respiratory activity.
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