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  • Title: Fast isolation of recombinant baculovirus by antibody screening.
    Author: Manns A, Grosse F.
    Journal: Biotechniques; 1991 Feb; 10(2):154-8. PubMed ID: 1711869.
    Abstract:
    The use of a rapid and efficient scheme for the detection and purification of recombinant baculoviruses is described. The method is based on the detection of foreign proteins in cellular lysates of baculovirus-infected insect cells by antibody screening. The recombinant virus is purified by repeated serial dilutions. The method allows the identification and purification of recombinant viruses within two to three weeks.
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