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  • Title: RP-HPLC prefractionation and its application in expressional proteomics analysis of an in vitro viral infection model.
    Author: Vaziri B, Rahimpour M, Eslami N, Fayaz A, Rahimian H.
    Journal: J Sep Sci; 2006 Oct; 29(15):2284-91. PubMed ID: 17120811.
    Abstract:
    Prefractionation of complex protein mixtures is an efficient method for increasing the separation power of 2-DE. RP-HPLC has been successfully utilized as a prefractionation method prior to 2-DE. Here we describe the optimization of an efficient RP-HPLC method for prefractionation of baby hamster kidney cell solubilized proteins. A step gradient elution of acetonitrile was optimized and collected fractions were further examined by SDS-PAGE and 2-DE. By utilizing this method an effective increase in separation power of 2-DE is accomplished. Moreover, we describe the application of this method to expressional proteome analysis of a virally infected cell model.
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