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  • Title: [Characterization of the mRNA profile in ejaculated spermatozoa from healthy fertile men].
    Author: Zhao YX, Li QL, Wang ZX, Wang YF, Wang LY, Qiao ZD.
    Journal: Zhonghua Nan Ke Xue; 2006 Oct; 12(10):900-3. PubMed ID: 17121019.
    Abstract:
    OBJECTIVE: To explore the complexity of mRNA in the ejaculated sperm from healthy fertile men. METHODS: Semen samples were collected from 10 healthy fathers. The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis. RESULTS: A totle of 21 052 SAGE raw tags were sequenced from 877 clones and 2 712 unique tags that occurred at least twice in the library were given further analysis. 19.7% of the unique tags had no match in the existing SAGE map, representing novel genes. Molecular function analysis revealed 67% of unique tags related to protein binding or nucleic acid binding categories, 41% to catalytic activity, 13% to message transducer activity, and 10% to transporter, structural and transcription regulator activity, respectively. CONCLUSION: There exists a complex repertoire of mRNAs in the ejaculated spermatozoa from fertile men.
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