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Title: [Establishment of miniCTTA multiplex set with fluorescence-labeled primers]. Author: Liu C, Wang HP, Sun HY, Wang HJ. Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2006 Nov; 26(11):1574-6. PubMed ID: 17121703. Abstract: OBJECTIVE: To establish a miniCTTA multiplex set including short tandem repeat (STR) markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp. METHODS: The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens. RESULTS: The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit. CONCLUSION: MiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.[Abstract] [Full Text] [Related] [New Search]