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  • Title: Development and validation of a competitive ELISA kit for the serological diagnosis of ovine, caprine and bovine brucellosis.
    Author: Portanti O, Tittarelli M, Di Febo T, Luciani M, Mercante MT, Conte A, Lelli R.
    Journal: J Vet Med B Infect Dis Vet Public Health; 2006 Dec; 53(10):494-8. PubMed ID: 17123429.
    Abstract:
    A competitive ELISA (Brucella-Ab c-ELISA) was standardized and validated for the detection of Brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (MAb 4B5A) produced against Brucella melitensis biotype 2. The specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (B/Bo%). When compared with an indirect ELISA, the Brucella-Ab c-ELISA did not demonstrate cross-reactions when testing positive sera for antibodies to some Enterobacteriaceae. A comparison was made between the Brucella-Ab c-ELISA and the complement fixation and Rose Bengal tests. Results demonstrated that the Brucella-Ab c-ELISA is a valuable tool for the serological diagnosis of bovine and ovine/caprine brucellosis.
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