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  • Title: Translation elongation factor 1-alpha gene from Pichia pastoris: molecular cloning, sequence, and use of its promoter.
    Author: Ahn J, Hong J, Lee H, Park M, Lee E, Kim C, Choi E, Jung J, Lee H.
    Journal: Appl Microbiol Biotechnol; 2007 Mar; 74(3):601-8. PubMed ID: 17124582.
    Abstract:
    The gene encoding translation elongation factor 1-alpha from the yeast Pichia pastoris was cloned. The gene revealed an open reading frame of 1,380 bp with the potential to encode a polypeptide of 459 amino acids with a calculated mass of 50.1 kDa. The potential of the promoter (P (TEF1)) in P. pastoris was investigated with comparison to the glyceraldehyde-3-phosphate dehydrogenase promoter (P (GAP)) by using a bacterial lipase gene as a reporter gene. P (TEF1) demonstrated a tighter growth-associated expression mode, improved functioning in the presence of high glucose concentrations, and promoter activities that yielded recombinant protein at levels similar to or in one case greater than P (GAP). The sequence of the gene was deposited in GenBank under accession no. EF014948.
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