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  • Title: A satellite RNA of barley yellow dwarf virus contains a novel hammerhead structure in the self-cleavage domain.
    Author: Miller WA, Hercus T, Waterhouse PM, Gerlach WL.
    Journal: Virology; 1991 Aug; 183(2):711-20. PubMed ID: 1713001.
    Abstract:
    An RNA molecule with properties of a satellite RNA was found in an isolate of barley yellow dwarf virus (BYDV), RPV serotype. It is 322 nucleotide long, single-stranded, and does not hybridize to the viral genome. Dimers of the RNA, which presumably represent replicative intermediates, were able to self-cleave into monomers. In vitro transcripts from cDNA clones were capable of self-cleavage in both the plus (encapsidated) and minus orientations. The sequence flanking the minus strand cleavage site contained a consensus "hammerhead" structure, similar to those found in other self-cleaving satellite RNAs. Although related to the hammerhead structure, sequences flanking the plus strand termini showed differences from the consensus and may be folded into a different structure containing a pseudo-knot.
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