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  • Title: Organophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection.
    Author: Karnati C, Du H, Ji HF, Xu X, Lvov Y, Mulchandani A, Mulchandani P, Chen W.
    Journal: Biosens Bioelectron; 2007 May 15; 22(11):2636-42. PubMed ID: 17140787.
    Abstract:
    We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic range extending from 10(-7) to 10(-3)M. The lower detection limit of approximately 10(-7)M for paraoxon was an order of magnitude better than the OPH-based potentiometric and optical biosensors based on pH modulation. There was a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard errors of 5% and 15%, respectively. OPs measured using this technique included parathion and diisopropyl fluorophosphate (DFP) in the order of paraoxon>DFP>parathion. The conformational change of the OPH was most likely the main origin of MCL bending.
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