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  • Title: [Methodological approaches to investigation of Na+-Ca2+ exchange in exocrine secretory cells].
    Author: Man'ko VV.
    Journal: Ukr Biokhim Zh (1999); 2006; 78(1):43-62. PubMed ID: 17147267.
    Abstract:
    An analysis of the methodological approaches, that used for investigation of Na+-Ca2+ exchange through the plasma membrane of exciting and secretory cells was presented in this review. Special attention is devoted to identification of Na+-Ca2+ exchange in the model for investigation of Ca2+ transporting systems of secretory cells - salivary glands of Chironomus plumosus L. larvae. With the aim different methods were used: researching of voltage-activated Ca2+-current depending on sodium gradient; studying of changes in the response of secretory glands, incubated in hypo- and hypersodium mediums, and Ca2+ content in their tissues; registration of Na+-Ca2+ exchange current in response to membrane hyper- or depolarisation changes of the membrane potential. And the current dependence on sodium and calcium ion gradient was also studied.
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