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  • Title: Aptamer-capped nanocrystal quantum dots: a new method for label-free protein detection.
    Author: Choi JH, Chen KH, Strano MS.
    Journal: J Am Chem Soc; 2006 Dec 13; 128(49):15584-5. PubMed ID: 17147356.
    Abstract:
    We demonstrate that aptamer-capped near-infrared PbS quantum dots (QDs) can detect a target protein based on selective charge transfer. The water-soluble QDs are synthesized with the thrombin-binding aptamer, which retains the secondary quadruplex structure necessary for binding to thrombin. These QDs have diameters of 3-6 nm and fluoresce around 1050 nm. When the aptamer-functionalized QD binds to its target, a fluorescence quenching occurs due to charge transfer from amine groups on the protein to the QD. Thrombin is detected within 1 min with a detection limit of approximately 1 nM. This selective detection is observed even in the presence of high background concentrations of interfering negatively or positively charged proteins, suggesting that aptamer-capped QDs could be useful for label-free protein assays.
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