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  • Title: Functional analysis of factors involved in trans-translation.
    Author: Takada K, Takemoto C, Kawazoe M, Konno T, Matsuta N, Kurita D, Asano K, Shirouzu M, Yokoyama S, Muto A, Himeno H.
    Journal: Nucleic Acids Symp Ser (Oxf); 2005; (49):101-2. PubMed ID: 17150653.
    Abstract:
    trans-translation is a rescue system for stalled ribosomes to terminate the translation for recycling of ribosome and lead the incomplete protein on the stalled ribosome to degradation. In trans-translation, several proteins and RNAs are involved. tmRNA and SmpB are only factors known to be essential for trans-translation. EF-Tu and EF-G functions in translation and also are seemed to function in trans-translation. Ribosomal protein S1 is known to bind tmRNA. In this study, we cloned the gene of tmRNA from Thermus thermophilus and analyzed the functions of factors involved in trans-translation in vitro.
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