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  • Title: SNPs typing by base-discriminating fluorescence DNA probe.
    Author: Okamoto A, Tainaka K, Ochi Y, Saito I.
    Journal: Nucleic Acids Symp Ser (Oxf); 2005; (49):201-2. PubMed ID: 17150703.
    Abstract:
    For the development of a novel SNP typing method using BDF (base-discriminating fluorescent) nucleosides in biological samples, we examined the detection of the single base alteration in BRCA1 gene with PCR products amplified by an asymmetric PCR. A combination of PyU- and PyC-containing BDF probes clearly facilitates the discrimination of not only A/G homozygous samples but also heterozygous samples. The present SNP typing method with BDF probes is a very powerful homogeneous assay that does not require a special device or time-consuming steps.
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