These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: Design of light-switchable phage promoter for efficient photo-regulation of gene-expression. Author: Liu M, Komiyama M, Asanuma H. Journal: Nucleic Acids Symp Ser (Oxf); 2005; (49):283-4. PubMed ID: 17150744. Abstract: Efficient photoregulation of transcription by T7 RNA polymerase (RNAP) has been achieved with modified T7-promoter involving two azobenzene moieties at RNAP recognition region and TATA region simultaneously. Transcription proceeded 7.5-fold faster after UV irradiation than that under dark condition. Kinetic parameters (kcat, Km) for the transcription revealed that an azobenzene at each position played a different role and thus synergistic effect of photo-regulation was achieved with the promoter involving two azobenzenes.[Abstract] [Full Text] [Related] [New Search]