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  • Title: Sensitive discrimination between cytosine and 5-methylcytosine in DNA by a modified invader method.
    Author: Yamada H, Tanabe K, Nishimoto S.
    Journal: Nucleic Acids Symp Ser (Oxf); 2006; (50):163-4. PubMed ID: 17150868.
    Abstract:
    We designed a detection system of 5-methylcytosine ((m)C) in DNA by a combination of photooxidative DNA cleavage reaction of 2-methyl-1,4-naphthoquinone chromophore with invader technology. Enzymatic treatment of a mixture of photochemically fragmented target oligodeoxynucleotides (ODNs) at (m)C and probe oligomer possessing a fluorophore and a quencher resulted in a dramatic enhancement of fluorescence. In contrast, fluorescence emission for the ODN containing C but not (m)C at the target sequence was extremely weak. We could detect (m)C in sub-femtomol DNA by monitoring the fluorescence change.
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