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  • Title: Enzymatic preparation of metabolic intermediates, 3-dehydroquinate and 3-dehydroshikimate, in the shikimate pathway.
    Author: Adachi O, Ano Y, Toyama H, Matsushita K.
    Journal: Biosci Biotechnol Biochem; 2006 Dec; 70(12):3081-3. PubMed ID: 17151445.
    Abstract:
    A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.
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