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  • Title: A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors.
    Author: Zhang H, Wu H, Zhang H.
    Journal: Biotechnol Lett; 2007 Mar; 29(3):431-7. PubMed ID: 17151958.
    Abstract:
    A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an L-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system.
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