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  • Title: Development of a liquid chromatography/electrospray ionization tandem mass spectrometric method for the determination of hydroxyl radical.
    Author: Yang F, Zhang R, He J, Abliz Z.
    Journal: Rapid Commun Mass Spectrom; 2007; 21(2):107-11. PubMed ID: 17154348.
    Abstract:
    A rapid method combining liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was developed for the determination of the hydroxyl radical (.OH). .OH generated via Fenton reaction was spin-trapped by 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and then analyzed by LC/ESI-MS/MS in multiple reaction monitoring (MRM) mode, using N-methyl-2-pyrrolidone (NMP) as the internal standard. The peak area ratio of DMPO-OH to NMP positively correlated with the concentration of .OH. The relative standard deviation (RSD) of the method was 1.13% (n = 8). The present method was successfully applied to evaluate the .OH scavenging capacity of several phenolic acids.
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