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  • Title: Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver.
    Author: Dráber P.
    Journal: Electrophoresis; 1991 Jun; 12(6):453-6. PubMed ID: 1716200.
    Abstract:
    A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate-sample buffer is described. Proteins are bound to a nitrocellulose membrane, stained with colloidal silver, and quantified by transmission densitometry. The staining requires 1 microL of protein sample and takes less than 20 min. Good linearity between the staining intensity and amount of proteins is in the range of 5-100 ng.
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