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  • Title: Functional properties of strychnine-sensitive glycine receptors expressed in Xenopus oocytes injected with a single mRNA.
    Author: Akagi H, Hirai K, Hishinuma F.
    Journal: Neurosci Res; 1991 Jun; 11(1):28-40. PubMed ID: 1716350.
    Abstract:
    Mature rat spinal cord cDNA libraries constructed in lambda gt10 and lambda ZAPII were screened with an oligonucleotide probe (39 mer), and 4 clones that possess DNA-inserts encoding a glycine receptor subunit were obtained. The cloned cDNAs were used to reconstruct the nucleotide sequence of the full-length open reading frame consisting of 1350 base pairs (bp) as well as the 5'-(184 bp) and 3'-(591 bp) non-coding regions. Synthetic RNA transcribed in vitro from the glycine receptor cDNA induced Xenopus oocytes to synthesize functional glycine receptor that generated large Cl- currents. The electrophysiological properties of the wild-type receptor and some mutant receptors produced by site-directed mutagenesis were analyzed.
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