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  • Title: Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes.
    Author: Gaul SB, Wedel S, Erdman MM, Harris DL, Harris IT, Ferris KE, Hoffman L.
    Journal: J Clin Microbiol; 2007 Feb; 45(2):472-6. PubMed ID: 17166969.
    Abstract:
    Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.
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