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  • Title: Unique 3'-untranslated sequence of insulin-like growth factor-I isolated from human placenta.
    Author: Daimon M, Wang CY, Johnson TR, Ilan J, Ilan J.
    Journal: Mol Reprod Dev; 1991 Jul; 29(3):238-44. PubMed ID: 1718331.
    Abstract:
    A cDNA clone of 525 bp corresponding to the 3'-untranslated region of insulin-like growth factor-I was isolated from a human placenta library. The sequence of this clone extended 200 nucleotides downstream from the previously reported 3'-end of IGF-IA cDNA, indicating the existence of IGF-IA transcripts having an even larger 3'-untranslated region. By using this clone for RNA transfer blot hybridization, it was shown that this longer 3'-untranslated region is included in the 7.5- and 5.0-kb transcripts, but not in the 1.1- and 0.9-kb transcripts. It is also apparent that transcripts bearing the extended 3'-untranslated sequence are highly expressed in human placenta.
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