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  • Title: [Identification of hydroxylate metabolites of daidzein and its sulfate conjugates in rat urine by LC-ESI/MS(n)].
    Author: Han FM, Ge BY, Chen HX, Chen Y.
    Journal: Yao Xue Xue Bao; 2006 Oct; 41(10):1004-9. PubMed ID: 17184121.
    Abstract:
    AIM: To identify the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine. METHODS: Urine samples from 0 - 24 h were collected after single ig dose of 500 mg x kg(-1) daidzein to each of six rats. The urine samples were purified by SPE column (SPE C18) and analyzed with liquid chromatographic-tandem electrospray ionization ion trap mass spectrometry (LC-ESI/MS(n)) for potential metabolites. RESULTS: Several new hydroxylate metabolites and its sulfate conjugates were found and identified in rat urine. CONCLUSION: LC-ESI/MS(n) is proved to be a simple, rapid, sensitive and specific technique for identification of the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.
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