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  • Title: Rapid species determination of Nocardia keratitis using pyrosequencing technology.
    Author: Galor A, Hall GS, Procop GW, Tuohy M, Millstein ME, Jeng BH.
    Journal: Am J Ophthalmol; 2007 Jan; 143(1):182-3. PubMed ID: 17188068.
    Abstract:
    PURPOSE: To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species. DESIGN: Interventional case report. METHODS: The medical records of a patient presenting with an infectious keratitis were reviewed. RESULTS: A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring. CONCLUSIONS: Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.
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