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  • Title: [The effect of preparative pancreatic digestion with collagenase on the glucose stimulated insulin secretion of isolated Langerhans islets].
    Author: Hahn HJ, Heinke P.
    Journal: Acta Biol Med Ger; 1975; 34(4):719-22. PubMed ID: 171895.
    Abstract:
    Pancreatic islets of wistar rats, isolated after 15 min of digestion with collagenase, secreted insulin in response to 15.0 mM glucose within 2 min and showed the typical sigmoidal glucose response during an incubation time of 15 and 60 min, respectively. Islets, isolated after 35 min of digestion with collagenase, responded with delay after stimulation with glucose (after 15 min of incubation), and are characterized by an increased "release" in the presence of 2.5 mM glucose.
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