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  • Title: Evaluation of first- and second-generation RIBA kits for detection of antibody to hepatitis C virus.
    Author: Chaudhary RK, MacLean C.
    Journal: J Clin Microbiol; 1991 Oct; 29(10):2329-30. PubMed ID: 1719025.
    Abstract:
    Samples reactive by first-generation recombinant immunoblot assay (RIBA) to detect antibody to hepatitis C virus (anti-HCV) (RIBA-1 [Chiron, Calif.]) remained reactive by a second-generation test (RIBA-2) for HCV antibodies. A total of 75% of specimens indeterminate by RIBA-1 became reactive, 12.5% were nonreactive, and 12.5% remained indeterminate by RIBA-2. Among RIBA-1-nonreactive specimens, 12.0% became positive and 5.1% became indeterminate by RIBA-2. The antigens c33c and c22-3 have increased the sensitivity of RIBA-2.
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