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  • Title: [Cloning preprolactin cDNA from Pacific salmon in Escherichia coli].
    Author: Prokopenko IV, Lomakin IB, Zakharova ES, Vinetskiĭ IuP.
    Journal: Mol Biol (Mosk); 1991; 25(3):689-94. PubMed ID: 1719371.
    Abstract:
    Prolactin coding mRNA was shown to be a prevalent part of chum salmon (Oncorhynchus keta) pituitary poly(A)-RNA during the spawning period. Clone lambda gtPrk12 was selected from the pituitary cDNA library by means of hybridization with the prolactin probe, and a nucleotide sequence of the insertion was determined and compared to the prolactin coding sequences from rainbow trout and Pacific chinook salmon, which had been published earlier. The sequences compared exhibited a significant homology. The deduced amino acid sequence of the chum salmon prolactin differed from a sequence determined directly in a single position. The prolactin-coding sequence can be used for constructing the bacterial strain producing prolactin.
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